SOLUTIONS

TSKgel SP-STAT

TSKgel SP-STAT 

TSKgel SP-STAT columns are packed with 7 and 10 µm nonporous resin particles of which the surface consists of long hydrophilic chains of open access network of multi-layered sulfopropyl cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.

Applications for the TSKgel SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.

Fast separation of protein standards 

sp-stat_fig1.png 
Columns:        A: TSKgel SP-STAT, 10 µm, 3.0 mm ID × 3.5 cm
                         B: ProSwift® SCX-1S Monolith, 4.6 mm ID × 5 cm
Mobile phase: A: 20 mmol/L sodium acetate, pH 5.0
                         B: 1.0 mol/L NaCl in mobile phase A, pH 5.0 for column A
                         1.5 mol/L NaCl in mobile phase A, pH 5.0 for column B
Gradient:        0 min (0%B) 1 min (100%B)
Flow rate:       A: 2.0 mL/min, B: 4.73 mL/min
Detection:       UV @ 280 nm
Samples:         1. α-chymotrypsinogen A
                         2. cytochrome C, 3. lysozyme  

 

Monitoring of reaction products 

sp-stat_fig2.png 
Column:          TSKgel SP-STAT, 10 µm, 3 mm ID × 3.5 cm
Mobile phase: A: 20 mmol/L sodium acetate buffer, pH 5.0
                         B: 1.0 mol/L NaCl in mobile phase A, pH 5.0
Gradient:        0 min (0%B) 2 min (100%B)
Flow rate:       2.0 mL/min
Detection:       UV @ 280 nm
Sample:          pegylated β-lactoglobulin 

 

Ordering Information

P/N Description Dimension
0021963 TSKgel SP-STAT 3 mm ID x 3.5 cm L
0021964 TSKgel SP-STAT 4.6 mm ID x 10 cm L