TSKgel Super-Octyl
TSKgel Super-Octyl columns are packed with monodispersed 2.3 μm spherical silica particles covalently bonded with C8 groups. It offers less hydrophobicity than TSKgel Super-ODS and is an excellent choice for peptides, proteins, amino acids, tryptic digests, nucleotides, pharmaceutical molecules, and food/beverage samples.
TSKgel Super-Octyl structure |
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Rapid separation of protein mixture |
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Column: TSKgel Super-Octyl, 2.3 µm, 4.6 mm ID × 5 cm L
Mobile phase: A: 13 mmol/L HCIO4,
B: 13 mmol/L HCIO4/CH3CN = 20/80
40% B to 100% B in a 1.5 min linear gradient
Flow rate: 2.0 mL/min
Detection: UV @ 220 nm
Samples: 1. ribonuclease A; 2. insulin; 3. cytochrome C; 4. lysozyme;
5. α-lactalbumin; 6. myoglobin |