TSKgel Super-ODS
TSKgel Super-ODS columns are packed with monodispersed 2.3 μm spherical silica particles covalently bonded with octadecyl groups. TSKgel Super-ODS is an excellent choice for small peptides, amino acids, tryptic digests, nucleotides, pharmaceutical molecules, and food/beverage samples.
Analysis of hydrophilic peptides |
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Column: TSKgel Super-ODS, 2.3 µm, 4.6 mm ID × 5 cm L
Mobile phase: 13 mmol/L HClO4/ACN Linear gradient
from 10% to 50% ACN over 10 minutes
Flow rate: 2 mL/min
Detection: UV @ 220 nm, micro--flow cell
Temperature: +25 °C
Samples: 1. oxytocin; 2. a-endorphin; 3. bombesin; 4. Leu-enkephalin;
5. gamma-endorphin; 6. somatostatin
All peptides were injected at 0.1 to 0.2 µg each. |
PTC amino acids |
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Column: TSKgel Super-ODS, 2.3 µm, 4.6 mm ID × 10 cm L
Mobile phase: A: ACN/50 mmol/L acetate buffer, pH 6.0 = 3/97
B: ACN/H2O = 60/40
Flow rate: 1.5 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Injection vol.: 5 mL (250 pmol)
Samples: 1. Asp 2. Glu 3. Ser 4. Gly 5. His 6. Arg 7. Thr 8. Ala 9. Pro
10. PTC-NH2 11. Try 12. Val 13. Met 14. Cys 15. Ile 16. Leu 17. Phe 18. Lys |