TOYOPEARL Ether-650
TOYOPEARL Ether-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with ether groups. TSKgel TOYOPEARL Ether-650 is the least hydrophobic TOYOPEARL resin and the best choice for the separation of very hydrophobic proteins or monoclonal antibodies.
TOYOPEARL Ether-650 is available in three particle sizes, 35 µm (S-grade), 65 µm (M-grade), and 100 µm (C-grade). This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL Ether-650 resins
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
65 µm (M-grade)
35 µm (S-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Typical dynamic binding capacities for humanized mAb
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Binding capacitiy (mg/mL - gel)
(10% breakthrough)
Phenyl-650M 35.2
Butyl-650M 42.1
PPG-600M 44.8
Butyl-600M 48.5 |
Selectivity of TOYOPEARL HIC resins
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| Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen |