TOYOPEARL Hexyl-650
TOYOPEARL Hexyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with C6 groups. This resin has the highest hydrophobicity of the TOYOPEARL HIC resins. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL Hexyl-650 resins
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
100 µm (C-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Typical dynamic binding capacities for humanized mAb
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Binding capacitiy (mg/mL - gel)
(10% breakthrough)
Phenyl-650M 35.2
Butyl-650M 42.1
PPG-600M 44.8
Butyl-600M 48.5 |
Selectivity of TOYOPEARL HIC resins
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| Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen |