TOYOPEARL Phenyl-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with phenyl groups. Its pore size has been optimized for the purification of monoclonal antibodies. It has an intermediate hydrophobicity, which is slightly higher than the one of the 100 nm pore size TOYOPEARL Phenyl-650. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
TOYOPEARL Phenyl-600M is typically used for intermediate purification or polishing steps. It is a very high recovery and high dynamic capacity resin for both large and small proteins. When used as the polishing step up to an extra 5% yield may be seen compared to other Phenyl resins.
To see other products optimized for HIC purification of mAbs please see TOYOPEARL PPG-600 and TOYOEPARL Butyl-600 media.
Structure of TOYOPEARL Phenyl-600 resins
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Product attributes
| Pore size (mean): |
75 nm |
| Particle size (mean): |
65 µm (M-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Comparison of TOYOPEARL-600M resins
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Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-600M
C. TOYOPEARL Butyl-600M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Samples: 1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL |
Breakthrough curves of polyclonal IgG on various HIC resins
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Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-600M
D. Competitor Phenyl Agarose
E. TOYOPEARL Phenyl-600M
Column size: 7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate: 300 cm/hr (2.4 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Samples: 1.0 g/L polyclonal IgG
DBC was calculated at 10% breakthrough |