TOYOPEARL PPG-600M
TOYOPEARL PPG-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with polypropylene glycol groups. It has the second lowest hydrophobicity of the HIC ligands offered by Tosoh Bioscience. Its pore size has been optimized for the purification of monoclonal antibodies. It is typically used for intermediate purification or polishing steps. It has extremely good capacity and recovery for mAbs.
This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL PPG-600M resins
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Product attributes
| Pore size (mean): |
75 nm |
| Particle size (mean): |
65 µm (M-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Comparison of TOYOPEARL -600M resins
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Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-600M
C. TOYOPEARL Butyl-600M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Samples: 1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL |
Breakthrough curves of polyclonal IgG on various HIC resins
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Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-600M
D. Competitor Phenyl Agarose
E. TOYOPEARL Phenyl-600M
Column size: 7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate: 300 cm/hr (2.4 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Samples: 1.0 g/L polyclonal IgG
DBC was calculated at 10% breakthrough |