TSKgel Ether-5PW
TSKgel Ether-5PW is based on a highly crosslinked 100 nm (1000 Å) pore size polymethacrylate base material bonded with polyether groups. TSKgel Ether-5PW provides the lowest hydrophobicity of the five HIC ligands offered by Tosoh Bioscience. It is useful for the separation of very hydrophobic molecules such as membrane proteins. It is typically used for high performance chromatography and polishing steps. It has the same selectivity as the larger particle TOYOPEARL Ether-650 products.
TSKgel Ether-5PW is less hydrophobic than Phenyl-5PW and the best choice for the separation of very hydrophobic proteins or monoclonal antibodies.
Structure of TSKgel Ether-5PW resins
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
30 µm
20 µm |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Purification of mAbs from mouse ascites fluid on TSKgel Ether-5PW and TOYOPEARL Ether-650M
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Resins: A: TSKgel Ether-5PW (prepacked HPLC column)
B: Toyopearl Ether-650M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.5 mol/L (NH4)2SO4 + 0.1mol/L phosphate, pH 7.0
Buffer B: 0.1 mol/L phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: A: 1.5 mg/100 µL anti-chicken 14 kDa lectin
B: 0.76 mg/50 µL diluted ascites fluid
Load volume: 150 µL |