TSKgel Phenyl-5PW
TSKgel Phenyl-5PW is based on a highly crosslinked 100 nm (1000 Å) pore size polymethacrylate base material bonded with phenyl groups. The TSKgel Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.
TSKgel Phenyl-5PW is less hydrophobic than Ether-5PW and the best choice for the separation of proteins with low hydophobicity.
Structure of TOYOPEARL Phenyl-5PW resins
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
20 µm
30 µm |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Typical dynamic binding capacities for humanized mAb
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Binding capacitiy (mg/mL - gel)
(10% breakthrough)
Phenyl-650M 35.2
Butyl-650M 42.1
PPG-600M 44.8
Butyl-600M 48.5 |
Selectivity of TOYOPEARL HIC resins
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| Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen |