TOYOPEARL CM-650
TOYOPEARL CM-650 is a weak cation exchange resin composed of polymethacrylate beads that have been functionalized with carboxymethyl (CM) weak cation exchange groups. TOYOPEARL CM-650 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.
TOYOPEARL CM-650 resins can be used for high throughput capture, intermediate purification, and polishing process steps. It is available in three particle sizes: 100 µm (C-grade), 65 µm (M-grade), and 35 µm (S-grade). TOYOPEARL CM-650 exhibits typical dynamic binding capacities for lysozyme of 40 g/L (S-grade, M-grade) and 35 g/L (C-grade).
Structure of TOYOPEARL CM-650
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
100 µm (C-grade)
65 µm (M-grade)
35 µm (S-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
Breakthrough curves of mono-PEGylated lysozyme using TOYOPEARL cation exchange resins
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Resins: A. TOYOPEARL SP-650M B. TOYOPEARL CM-650M
C. TOYOPEARL SP-550C D. TOYOPEARL GigaCap CM-650M
E. TOYOPEARL GigaCap S-650M
Column size: 6 mm ID × 40 mm
Mobile phase: Buffer A: 20 mmol/L phosphate buffer, pH 7.0
Buffer B: 20 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mono-PEGylated lysozyme, 1.0 mg/mL (PEG MW= 5 kDa)
Dynamic binding capacities were determined at 10% breakthrough |
Separation of monoclonal antibody cell culture supernatant
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Resin: TOYOPEARL CM-650S
Column size: 16 mm ID × 6 cm
Mobile phase: Buffer A: 20 mmol/L sodium acetate, pH 5.5
Buffer B: 20 mmol/L sodium acetate, pH 5.5 + 0.5 mol/L NaCl
Gradient: linear gradient from buffer A to buffer B in 200 mL total volume
Flow rate: 173 cm/hr (5.8 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 100 mL of monoclonal antibody cell culture supernatant |