TOYOPEARL DEAE-650
TOYOPEARL DEAE-650 is a weak anion exchange resin for biomolecule purification. It is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups. TOYOPEARL DEAE-650 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.
TOYOPEARL DEAE-650 can be used for high throughput capture, intermediate purification, and polishing process steps. It is frequently used in plasma fractionation and for the purification of recombinant blood coagulation factors.
TOYOPEARL DEAE-650 is available in three particle sizes: 100 µm (C-grade), 65 µm (M-grade), and 35 µm (S-grade). If greater resolution is needed, the smaller particle TSKgel DEAE-5PW resin may be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand, the selectivity will be unchanged.
Structure of TOYOPEARL DEAE-650
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
100 µm (C-grade)
65 µm (M-grade)
35 µm (S-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
pKa values for ion exchange groups
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Selectivity comparisons of anion exchange resins
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Resins: A. TOYOPEARL GigaCap Q-650S
B. Competitor Product
C. Competitor Product
D. TOYOPEARL SuperQ-650S
E. TSKgel SuperQ-5PW (30)
F. TSKgel SuperQ-5PW (20)
G. TOYOPEARL DEAE-650S
H. TSKgel DEAE-5PW (30)
I. TSKgel DEAE-5PW (20)
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 50 mmol/L Tris-HCl, pH 8.5
Buffer B: buffer A + 1.0 mol/L NaCl
Gradient: 0-100% buffer B (120 min)
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Injection vol.: 100 µL
Samples: transferrin, 2.9 g/L
ovalbumin, 6.5 g/L
trypsin inhibitor, 10.0 g/L |
Separation of human serum albumin fractions
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Resins: 1. TOYOPEARL DEAE-650S
2. TOYOPEARL DEAE-650MColumn size: 16 mm ID × 15 cm
Mobile phase: Buffer A: 50 mmol/LTris-HCl, pH 8.6 Buffer B: 50 mmol/L Tris-HCl,
pH 8.6 + 0.5 mol/L NaCl
Gradient: linear gradient from buffer A to buffer B in 200 mL total volume
Flow rate: 45 cm/hr (1.5 mL/min)
Detection: UV @ 280 nm
Temperature: 25 ˚C
Sample: human serum
Column: TSKgel G3000SW, 7.5 mm ID × 30 cm × 2 in series
Mobile phase: 0.1 mol/L phosphate, pH 6.8 + 0.1 mol/L sodium sulfate
Detection: UV @ 280 nm
Temperature: 25 ˚C
Sample: 1. crude human serum
2. albumin fraction from TOYOPEARL DEAE-650S
3. albumin fraction from TOYOPEARL DEAE-650M
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