TOYOPEARL GigaCap CM-650
TOYOPEARL GigaCap CM-650 is a high capacity cation exchange resin optimized for the high throughput purification of IgG’s. It is composed of polymethacrylate beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with carboxymethyl (CM) weak cation exchange groups.
TOYOPEARL GigaCap CM-650 exhibits different selectivity than the TOYOPEARL GigaCap S-650 resin. This difference, combined with its unusually high capacity for a weak cation exchange resin, may be exploited for wither mAb or traditional protein purifications. TOYOPEARL GigaCap CM-650 resin is available in 75 µm particle size, which corresponds to the M-grade designation for TOYOPEARL resins.
TOYOPEARL GigaCap CM-650M exhibits typical dynamic binding capacities of 100 g/L for human IgG and maintains its high capacity at increased linear velocities. It has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step. TOYOPEARL GigaCap CM-650 media also has excellent caustic stability in 0.5 mol/L NaOH. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL GigaCap CM-650M
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Product attributes
| Pore size (mean): |
< 100 nm |
| Particle size (mean): |
75 µm (C-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
3-13 |
| Stability data: |
10 years (estimated) |
TOYOPEARL GigaCap CM-650M has unique selectivity
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Resins: TOYOPEARL GigaCap CM-650M TOYOPEARL GigaCap S-650M
Column size: 6 mm ID × 4 cm
Mobile phase: Buffer A: 20 mmol/L phosphate, pH 7.0
Buffer B: 20 mmol/L phosphate + 1.0 mol/L NaCl, pH 7.0
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Injection vol.: 25 µL
Samples: (A) 1. ribonuclease A (5.0 g/L) 2. cytochrome C (1.9 g/L)
3. lysozyme (3.8 g/L)
(B) 1. trypsinogen (3.8 g/L) 2. ribonuclease A (5.0 g/L)
3. lysozyme (3.8 g/L) |
Selectivity comparison between native protein and mono-PEGylated protein on TOYOPEARL GigaCap resins
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Resins: TOYOPEARL GigaCap S-650M TOYOPEARL GigaCap CM-650M
Column size: 6 mm ID × 4 cm
Mobile phase: Buffer A: 50 mmol/L phosphate buffer, pH 7.0
Buffer B: 50 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Gradients: 10 minute 100% buffer A 10 minute 100% buffer A 60 minutes
0%B to 100%B 60 minutes 0%B to 50%B
Flow rate: 212 cm/hr (1 mL/min)
Samples: native lysozyme, 5 g/L mono-PEGylated lysozyme, 5 g/L
(PEG MW= 5 kDa) |