TOYOPEARL SP-550
TOYOPEARL SP-550 is a strong cation exchange resin for biomolecule purification. It is composed of polymethacrylate beads that have been functionalized with Sulfopropyl (SP) strong cation exchange groups. TOYOPEARL SP-550 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.
TOYOPEARL SP-550 is available 100 µm particle size (C-grade), designed to be used for high throughput capture of product from biological feedstock. It exhibits typical dynamic binding capacities for lysozyme of 100 g/L. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL SP-550
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Product attributes
| Pore size (mean): |
< 50 nm |
| Particle size (mean): |
100 µm (C-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
Breakthrough curves of mono-PEGylated lysozyme using TOYOPEARL cation exchange resins
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Resins: A. TOYOPEARL SP-650M B. TOYOPEARL CM-650M
C. TOYOPEARL SP-550C D. TOYOPEARL GigaCap CM-650M
E. TOYOPEARL GigaCap S-650M
Column size: 6 mm ID × 40 mm
Mobile phase: Buffer A: 20 mmol/L phosphate buffer, pH 7.0
Buffer B: 20 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mono-PEGylated lysozyme, 1.0 mg/mL (PEG MW= 5 kDa)
Dynamic binding capacities were determined at 10% breakthrough |