TSKgel DEAE-5PW
TSKgel DEAE-5PW is a weak anion exchange resin for biomolecule purification. The resin is composed of highly crosslinked polymethacrylate beads that have been functionalized with tertiary amine groups, namely diethylaminoethyl groups. TSKgel DEAE-5PW resins are best used for high resolution process polishing steps. They are available in two particle sizes: 30 µm and 20 µm.
TSKgel DEAE-5PW resins have high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography.
Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.
Structure of TSKgel DEAE-5PW
|
 |
Product attributes
| Pore size (mean): |
< 100 nm |
| Particle size (mean): |
30 µm
20 µm |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
Selectivity Comparison of Anion Exchange Resins
|
 |
Resins: A. TOYOPEARL GigaCap Q-650S B. Competitor Q C. Competitor Q D. TOYOPEARL SuperQ-650S E. TSKgel SuperQ-5PW (30) F. TSKgel SuperQ-5PW (20) G. TOYOPEARL DEAE-650S H. TSKgel DEAE-5PW (30) I. TSKgel DEAE-5PW (20)
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 50 mmol/L Tris-HCl, pH 8.5
Buffer B: buffer A + 1.0 mol/L NaCl
Gradient: 0-100% buffer B (120 min)
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Injection vol.: 100 µL
Samples: transferrin, 2.9 g/L ovalbumin, 6.5 g/L trypsin inhibitor, 10.0 g/L |