TSKgel SP-3PW
TSKgel SP-3PW is a strong cation exchange resin for biomolecule purification. It is composed of highly cross-linked polymethacrylate beads that have been functionalized with sulfopropyl (SP) strong cation exchange groups.
TSKgel SP-3PW resins are best used for high resolution process polishing steps for peptide purifications such as insulin. TSKgel SP-3PW resin is available in 30 µm particle sizes, and has a smaller pore size (25 nm) than the TSKgel SP-5PW (100 nm).
Structure of TSKgel SP-3PW |
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Selectivity Comaprison |
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Column: 7.5 mm ID x 7.5 cm L
Mobile phase: A: 20 mM sodium citrate buffer (pH 3.2)/ethanol = 8/2 (v/v);
B: 1.0 mol/L NaCl in 20 mM sodium citrate buffer
(pH 3.2)/ethanol = 8/2 (v/v)
Gradient: 60 min linear gradient from Buffer A to Buffer B
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: RT
Sample: 1. trypsinogen, 2. insulin, 3. lysozyme; 100 µL (0.5 mg/ml each)
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