TSKgel SP-5PW
TSKgel SP-5PW is a strong cation exchange resin for biomolecule purification. It is composed of highly crosslinked polymethacrylate beads that have been functionalized with sulfopropyl (SP) strong cation exchange groups. TSKgel SP-5PW resins are available in two particle sizes: 30 µm and 20 µm, and ideally suited for high resolution polishing steps. The TSKgel SP-5PW (20) resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TSKgel SP-5PW
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
30 µm
20 µm |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
Selectivity Comparison
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Column: 7.5 mm ID x 7.5 cm L
Mobile phase: A: 20 mM sodium citrate buffer (pH 3.2)/ethanol = 8/2 (v/v);
B: 1.0 mol/L NaCl in 20 mM sodium citrate buffer
(pH 3.2)/ethanol = 8/2 (v/v)
Gradient: 60 min linear gradient from Buffer A to Buffer B
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: RT
Sample: 1. trypsinogen, 2. insulin, 3. lysozyme; 100 µL (0.5 mg/ml each)
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