TSKgel SuperQ-5PW
TSKgel SuperQ-5PW is a strong anion exchange resin for biomolecule purification. It is composed of highly crosslinked polymethacrylate beads that have been functionalized with quaternary amine (Q) strong anion exchange groups.
TSKgel SuperQ-5PW has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography.
TSKgel SuperQ-5PW resins are best used for high resolution process polishing steps and the capture of oligonucleotides from crude harvest. TSKgel SuperQ-5PW is available in two particle sizes: 30 µm and 20 µm. This resin is also available in pre-packed SkillPak columns to simplify your process developmentand scale up.
Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.
Product attributes
| Pore size (mean): |
< 100 nm |
| Particle size (mean): |
30 µm
20 µm |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
pKa values for Ion Exchange groups
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TSKgel SuperQ-5PW (20) maintains resolution at high oligonucleotide load
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Column: 0.66 cm x 15 cm L (5.1 mL) (resin as noted in figure)
Flow rate: 1.43 mL/min (250 cm/h)
Buffer A: 20 mmol/L Tris-HCl + 10 mmol/L EDTA (pH= 9.0)
Buffer B: 20 mmol/L Tris-HCl + 10 mmol/L EDTA + 1.0 mol/L NaCl (pH= 9.0)
Sample loaded: DNA based oligonucleotides were loaded as followed: 1 mg/column panels A & B, 20 mg/column panels C & D
Separation conditions: Column was washed with 5 CV 100 % buffer A followed by 11 mL injection. Column was then washed with 3 CV 100 % buffer A followed by 6 CV of linear gradient 35-53 buffer B. Finally, column was washed with 5 CV 100 % buffer B.
Detection: UV @ 254 nm
Fractions: 0.5 mL fractions were taken from peaks of interest and analyzed on a TSKgel DNA-NPR column
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TSKgel SuperQ-5PW (20), 1.0 mg load
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Resin: TSKgel SuperQ-5PW (20)
Column size: 6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient: 50% B (2 CV) 50-100% B (15 CV) 100% B (2 CV)
Flow rate: 200 cm/hr (1.14 mL/min)
Detection: UV @ 254 nmSample: crude phosphorothioate deoxyoligonucleotide
Sample load: 1.0 mg
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