TOYOPEARL AF-rProtein A-650F
TOYOPEARL AF-rProtein A-650F is an alkaline resistant protein A affinity resin developed for the capture and purification of monoclonal antibodies. TOYOPEARL AF-rProtein A-650F utilizes an alkaline resistant recombinant Protein A variant. This non-animal derived ligand is bound to the base bead using multipoint attachment. This contributes to both the resin's alkaline resistance and its low ligand leakages in the range of 5-25 ng/mg IgG. Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at short residence times.
TOYOPEARL AF-rProtein A-650F exhibits typical dynamic binding capacities of ≥30 g/L for human IgG at 2 minutes residence time and maintains this capacity at increased linear velocities. The resin maintains its capacity well after repeated CIP exposure to 0.1mol/L NaOH and has reasonable tolerance to CIP with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A-650F also tolerates exposure to above ambient temperatures. The resin is available in 45 µm F-grade particle size.
Structure of TOYOPEARL AF-rProtein A-650F
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
45 µm (F-grade) |
| Shipping buffer: |
20% ethanol |
| SBC: |
> 45 g/L |
| DBC: |
> 30 g/L |
Comparison of linear velocity and pressure curves
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Resins: A. TOYOPEARL AF-rProtein A -650F, 45 µm, 20 cm ID × 32 cm
B. TOYOPEARL AF-rProtein A -650F, 45 µm, 20 cm ID × 18 cm
C. TOYOPEARL Butyl-650M, 65 µm, 20 cm ID × 21 cm
Column: QuikScale®, 20 cm ID
Mobile phase: H2O
Linear velocity: various
Detection: pressure (MPa) |
Temperature stability
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Resin: TOYOPEARL AF-rProtein A-650F
Mobile phase: deionized H2O
Autoclave settings: 120 °C, 1.2 atm, 15 min
Heating time: 30 min
TOYOPEARL AF-rProtein is stable at 35 °C for least 3 years (data not shown) |