TOYOPEARL HW-40
TOYOPEARL HW-40 is a hydroxylated methacrylic polymer with a small pore size of 5 nm (50 A) for size exclusion chromatography of biomolecules. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.
TOYOPEARL HW-40 is available in three particle sizes, 30 µm (S-grade), 45 µm (F-grade), and 75µm (C-grade). This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL HW Resins
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Comparison of resolution on different particle sizes of TOYOPEARL HW-55 resin
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Resin: TOYOPEARL HW-55
Column size: 26 mm ID × 70 cm
Mobile phase: 33.3 mmol/L phosphate buffer, pH 7.0, 0.2 mol/L NaCI
Flow rate: 20 cm/hr (1.77 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 1 mL
Samples: 1. thyroglobulin (0.3%), 2. gamma-globulin (0.3%),
3. β-lactoglobulin (0.3%), 4. cytochrome C (0.1%) |