TOYOPEARL HW-50
TOYOPEARL HW-50 is a hydroxylated methacrylic polymer with a pore size of 12.5 nm (125 A) for size exclusion chromatography of biomolecules between 500-80,000 Da.
TOYOPEARL HW-50 is available in two particle sizes, 30 µm (S-grade) and 45 µm (F-grade)
Structure of TOYOPEARL HW Resins |
|
Comparison of resolution on different particle sizes of TOYOPEARL HW-55 resin |
|
Resin: TOYOPEARL HW-55
Column size: 26 mm ID × 70 cm
Mobile phase: 33.3 mmol/L phosphate buffer, pH 7.0, 0.2 mol/L NaCI
Flow rate: 20 cm/hr (1.77 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 1 mL
Samples: 1. thyroglobulin (0.3%), 2. gamma-globulin (0.3%),
3. β-lactoglobulin (0.3%), 4. cytochrome C (0.1%)
|