TOYOPEARL HW-65
TOYOPEARL HW-65 is a hydroxylated methacrylic polymer with a pore size of 100 nm (1000 A) for size exclusion chromatography of biomolecules between 40,000-5,000,000 Da. It also is the base bead for all TOYOPEARL"-650" designated products.
TOYOPEARL HW-65 is available in three particle sizes, 30 µm (S-grade), 45 µm (F-grade), and 100 µm (C-grade).
Structure of TOYOPEARL HW Resins
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Comparison of resolution on different particle sizes of TOYOPEARL HW-55 resin
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Resin: TOYOPEARL HW-55
Column size: 26 mm ID × 70 cm
Mobile phase: 33.3 mmol/L phosphate buffer, pH 7.0, 0.2 mol/L NaCI
Flow rate: 20 cm/hr (1.77 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 1 mL
Samples: 1. thyroglobulin (0.3%), 2. gamma-globulin (0.3%),
3. β-lactoglobulin (0.3%), 4. cytochrome C (0.1%) |